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      [KO Validated] N-Cadherin Rabbit mAb

      時(shí)間:2020-10-29 09:57   瀏覽次數(shù):1775次

      品牌 ABclonal 產(chǎn)品類型 抗體
      產(chǎn)品名稱 [KO Validated] N-Cadherin Rabbit mAb 產(chǎn)品規(guī)格 50ul /100ul /200ul
      產(chǎn)品目錄號 A19083 產(chǎn)品目錄價(jià) 800/1200/2000

      背景

      This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

      免疫原信息

      應(yīng)用

      反應(yīng)物種Human, Mouse, Rat
      驗(yàn)證應(yīng)用WBIHCIF
      推薦稀釋比&實(shí)驗(yàn)步驟
      • WB   1:500 - 1:2000  實(shí)驗(yàn)步驟 ]
      • IHC   1:50 - 1:200  實(shí)驗(yàn)步驟 ]
      • IF   1:50 - 1:200  實(shí)驗(yàn)步驟 ]
      理論分子量140kDa
      實(shí)際分子量140kDa
      存儲緩沖液Store at -20℃. Avoid freeze / thaw cycles.
      Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
      實(shí)驗(yàn)方法Western blotting    
      陽性樣本HeLa,293T,NIH/3T3,C6
      細(xì)胞定位
      純化方式Affinity purification

      [KO Validated] N-Cadherin Rabbit mAb 檢測圖片

      ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

      Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

      Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
      Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
      Lysates/proteins: 25ug per lane.
      Blocking buffer: 3% nonfat dry milk in TBST.
      Detection: ECL Basic Kit (RM00020).
      Exposure time: 1min.
      ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

      Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

      Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
      Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
      Lysates/proteins: 25ug per lane.
      Blocking buffer: 3% nonfat dry milk in TBST.
      Detection: ECL Basic Kit (RM00020).
      Exposure time: 1min.
      ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

      Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

      Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
      ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

      Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

      Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
      ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

      Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

      Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.


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